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Mortimer Benioff was born in 1912 to Anna J. Chlebniaov and George Benioff. He was the brother of Ralph Benioff (1913-1929) and William Julian Benioff (1919-1968). He married Sophie Carolyn Prescott in 1938 in San Francisco and had a son in 1942 and a daughter in 1945.
Mortimer attended the University of California. At the California Medical Center in San Francisco, he was a clinical assistant in medicine. He received an AB degree and his MD.
His specialty was in diseases of the lung including tuberculosis. In 1968, Mortimer testified about smoke inhalation in a law suit brought by a woman whose husband died of lung cancer. His testimony helped to win the case bringing evidence that cancer-causing carcinogens like those found in cigarettes can also be found in the smoke from fires.